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. 2013 May 13;288(26):19260–19268. doi: 10.1074/jbc.M112.445270

FIGURE 6.

FIGURE 6.

Cpc2, but not snoU24b RNA, is important for Gcn2 regulation. A, small nucleolar RNA U24b is encoded within the intron of cpc2 mRNA. Gray box, coding sequence; dashed line, intron; solid line, untranslated region. The position encoding Trp43 is indicated by an arrowhead. B, gene expression levels of cpc2, snoU24b, and cdk9+ (control) were analyzed by quantitative RT-PCR from wild-type (YT3033), cpc2Δ (YT2307), and cpc2_W43* (YT4299) cells. All data are normalized to the expression level of 18 S rRNA, and the average expression level of two independent experiments is shown as the relative fold to those in wild-type cells. C, 10-fold serial dilutions of wild-type (JK317), cpc2Δ (YT3540), and cpc2_W43* (YT4299) cells were spotted on EMM plates ± 10 mm 3AT. D, immunoblots of anti-FLAG-Gcn2 immunoprecipitates from Flag:gcn2 (YT3372) and Flag:gcn2 cpc2_W43* (YT4309) cells treated with 10 mm 3AT, probed with anti-phospho-Gcn2 and anti-FLAG (control) antibodies.