Fig. 3.

Mammospheres are enriched for high tumorigenicity in vivo. Dissociated mammospheres and adherent cells were injected SC into NOD-SCID mice. The number of mice with a tumour was tabulated (A) and representative images of tumours 3 weeks after inoculation are shown (B). (C) Tumour volumes generated by spheres and adherent cells were measured and recorded 3 weeks after inoculation: 10⁴: ^*P < 0.04; 10⁶: ^**P < 0.001. (D) Haematoxylin and eosin staining of histological sections of representative xenograft tumour sections. Scale bar = 50 μm.