Fig. 3.

Ly-6G⁺ neutrophils produce IFN-γ. TLR11^−/− mice were infected i.p. with T. gondii and on day 5, cells were sort purified as shown, isolating Ly-6G⁺ CD3ε⁻ and NK1.1⁺ CD3ε⁻ cells from peritoneal exudate cells (PEC) (A). (B) Ly-6G⁺ cells (Left) and NK1.1⁺ cells (Center) were defined by histogram expression of IFN-γ (open histograms) compared with its appropriate isotype control (filled histogram) and percent of IFN-γ quantified in a bar graph (Right). (C) Sorted Ly-6G⁺ cells contain IFN-γ transcript as quantified by fold change over bulk naïve PEC by quantitative RT-PCR. (D) Sort-purified Ly-6G⁺NK1.1⁻ CD3ε⁻ PEC were analyzed morphologically by Giemsa staining. (E) Purified Ly-6G⁺ cells were analyzed for IFN-γ protein by staining with αIFN-γ antibody and analyzed by confocal microscopy. The data shown are one of three independent experiments, and error bars shown are the means ± SEM.