Fig. 4.

Functional interaction between SPIDR and RAD51. (A) 293T cells were transfected with plasmids encoding SFB-tagged RAD51 or Morc3 together with plasmids encoding Myc-tagged SPIDR. Coprecipitation and immunoblotting were carried out as indicated. (B) Direct binding between recombinant GST-RAD51 purified from bacteria and in vitro-translated SPIDR. (Upper) SPIDR was detected by immunoblotting. (Lower) Input GST-proteins visualized by Coomassie staining. (C and D) SiRNA-treated HeLa cells were lysed in the presence of benzonase, cell lysates were then incubated with protein A agarose beads conjugated with indicated antibodies, and Western blot analysis was carried out as indicated.