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. 2013 Jun 10;110(26):10866–10871. doi: 10.1073/pnas.1301647110

Fig. 2.

Fig. 2.

ABI5, RGA, and MFT are direct targets of SPT. (A and B) Relative transcript abundance (Rel. transc. abund.) in Ler and Col freshly matured dry seeds of selected genes involved in ABA and GA biosynthetic and signaling pathways, DOG1 and MFT. Error bars represent SD of three determinations. (C) Schematic representation of the 2,500-bp region upstream of the ATG start codon of the indicated genes: Vertical thick black bars represent G-box motifs; vertical thick gray bars represent E-box motifs in G-box free promoters; light gray rectangles indicate the presence of an upstream gene; horizontal thin black lines represent amplicons assayed for SPT binding. (D) Relative ChIP-qPCR enrichment (Rel. ChIP enrich.) of the indicated promoter regions compared with the negative MLS control. Assays were performed on silique samples from a SPTmyc epitope-tagged transgenic line (Ler) with (+Ab) or without (−Ab) anti-MYC antibodies. Error bars represent standard error (SE) of three replicates (similar results were obtained in independent experiments). Asterisks above the bars indicate statistically significant differences (P < 0.05). See Experimental Procedures for details.