Fig. 6.

cDC-derived IL-23 triggers innate IL-17/IL-22 production. IL-23p19^−/− mice were painted with IMQ for 2 consecutive days. (A) Relative expression of IL-23p19, IL-17A, and IL-22 in back skin of 2-d IMQ-treated mice was analyzed by quantitative RT-PCR. (B) Serum levels of IL-6, TNFα, and IL-22 were quantified by CBA. Bars represent mean ± SD of n ≥ 4 animals per group. (C) Production of IL-23p19 by different skin DC subsets was determined during the first 12 h and between 24 and 36 h after (repetitive) IMQ-painting. Cells were gated on CD45⁺ MHCII⁺ CD11c⁺ DCs. Representative FACS plots out of at least two experiments (n = 3 animals per group) are depicted. Bars represent mean ± SD of n ≥ 3 animals per group.