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. 2013 Jun 10;110(26):E2362–E2370. doi: 10.1073/pnas.1301837110

Fig. 1.

Fig. 1.

miR-125, let-7, and miR-9 are late-progenitor miRNAs. (A) Schematic representation of retinal development. ERP, early retinal progenitor; LRP, late retinal progenitor; RGC, retinal ganglion cell; CON, cone cell; HOR, horizontal cell; AMA, amacrine; ROD, rod photoreceptor; BIP, bipolar cells; MUL, Müller glia. (B) Heat map representation in a green-black-red gradient of miRNA with greatest increase between E14 and P0. (C) RT-qPCR analysis of FACS-sorted Hes5-GFP P0 retinas. Note the enrichment of miR-9, let-7f, and let-7a in progenitor cells. (D) RT-qPCR validation of expression profiles for miR-125, let7a, let7f, miR-9, and miR-183 across five developmental stages. Data indicate mean ± SEM. (E and F) Ganglion cells (Brn3+) increase in the Dicer CKO retina, and (G and H) a similar increase is seen with transfection of antagomirs to late progenitor miRNAs compared to controls. Scale bar, 100 microns.