Skip to main content
. 2013 Jun 10;110(26):E2362–E2370. doi: 10.1073/pnas.1301837110

Fig. 2.

Fig. 2.

miR-125, let-7, and miR-9 can rescue the Dicer CKO phenotype and accelerate the developmental timing. (A–D′′) LP-miRNA transfection in Dicer-CKO retinas rescued Ascl1 expression. (A and B) Ascl1 (white) was expressed in the developing retina of Dicer-HET controls (A) but was absent in Dicer-CKO transfected with nCherry (red) control construct (B). Coelectroporation of LP-miRNAs together with nCherry (red) rescued Ascl1 expression in Dicer-CKO retina (arrow in D). (E–H′′) LP-miRNA electroporation reduced the number ganglion cells in Dicer-CKO retinal explants. Ganglion cells (Brn3+; white) in Dicer-Het (E) and Dicer-CKO retinas (F–H′′). Coelectroporation of LP-miRNAs with nCherry (labeled with RFP, red) resulted in a reduction in the number of Brn3+ cells (G and H). (Scale bar: A–H′′, 100 μm.) Error bars indicate mean ± SEM. P values: **<0.01; ***<0.001. NBL, neuroblastic layer; GCL, ganglion cell layer. (I–Q) LP-miRNA transfection accelerates development in normal retina. Brn3 (I and J) and Ascl1 (L and M) in E14 retinas transfected with control mimics (I and L) or LP-miRNA (J and M) together with nCherry (red). (O–Q) LP-miRNAs increased the number of Nrl+ photoreceptors in Nrl-GFP E14 retinal explants (P and P′). Error bars indicate mean ± SEM. P values: *<0.05; ***<0.001.NBL, neuroblastic layer; GCL, ganglion cell layer.