Fig. 4.

Overexpression of Lin28 or Prtg maintains an early progenitor competence. (A–F) Prtg or Lin28 overexpression increased the number of ganglion cells generated in the neuroblastic layer. E16 wild-type retinas were electroporated with nCherry alone (A and A′) or together with Lin28 (B and B′; D and D′′) or Prtg (C and C′; E and E’) and labeled with Brn3 (green) and RFP (red) antibodies. Brn3+ ganglion cells increase in the neuroblastic layer after Prtg or Lin28 overexpression (arrows) compared with controls. (D–E′′) Labeling for EdU (red) colabeled Brn3+ ganglion cells (green) in the neuroblastic layer (arrows). (F) Graph of effects of Prtg or Lin28 overexpression; mean ± SEM. P values: ***<0.001. NBL, neuroblastic layer; GCL, ganglion cell layer; L, lenses. (Scale bars: A–C′, 200 μm; D–E′′, 100 μm.)