Fig. 2.

Kidney-specific transgenic overexpression of miR-17∼92 produces kidney cysts. (A) qRT-PCR analysis showing that the expression of miRNAs encoded by the miR-17∼92 miRNA cluster is up-regulated in kidneys from miR-17∼92 OE mice compared with control mice. (B) Expression of miRNAs encoded by the miR-17∼92 miRNA cluster is reduced in kidneys from miR-17∼92 KO mice compared with control mice. **P < 0.01, *P < 0.05. Error bars represent SEM. (C) H&E staining demonstrates normal kidney histology in control mice (a) and miR-17∼92KO mice (c). In contrast, kidney tubular cysts (cy, arrows) are present in 28-d-old miR-17∼92OE mice (b). Staining with Dolichos biflorus agglutinin (green, d, e, and f), a marker of collecting ducts; LTA (green, g, h, and i), a marker of proximal tubules; and THP (red, j, k, and l), a marker of loops of Henle; showed that the cysts in miR-17∼92 OE mice were derived from collecting ducts (e) and loops of Henle (k). No proximal tubular cysts were observed (h). (Scale bars: 40 μm.)