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. 2013 Jun 10;110(26):10574–10579. doi: 10.1073/pnas.1309211110

Fig. 2.

Fig. 2.

Fam20C activity and specificity are evolutionarily conserved. (A) Human Fam20C and ceFam20 phosphorylated the Fam20C model peptide substrate β28–40, whereas Fam20B did not. (B) Fam20B phosphorylated a model substrate representing the tetrasaccharide core linkage region in proteoglycans (GlcAβ1–3Galβ1–3Galβ1–4Xylβ1-O-benzyl), whereas Fam20C and ceFam20 did not. (C) Peptides containing variants of the Ser-x-Glu Fam20C consensus motif were synthesized to assess the specificity of ceFam20. The Ser was changed to Thr or Tyr, and the n+2 residue was changed to Asp. Incorporation of phosphate was monitored by autoradiography.