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. 2013 Jun 10;110(26):10640–10645. doi: 10.1073/pnas.1220662110

Fig. 5.

Fig. 5.

KIAA0146 is a binding partner of FIGNL1 and participates in homologous recombination repair. (A) TAP was performed using 293T cells stably expressing SFB-tagged FIGNL1. The data from MS analysis are shown. (B) Association of endogenous KIAA0146 with FIGNL1 in 293T cells was performed by coimmunoprecipitation. (C) KIAA0146 and FIGNL1 interact directly with each other. The main bands are indicated by arrows. The lower bands are caused by protein degradation. (D) FIGNL1 binds to the C terminus of KIAA0146. Schematic representation of the full-length and deletion mutants of KIAA0146 used in this study is shown. FL, full length. (E) Amino acids 121–240 are required for FIGNL1 binding to KIAA0146. (F) KIAA0146 depletion impairs HR repair. Data are presented as mean ± SD from three different experiments. (G) Amino acids 121–240 deletion mutant could not rescue HR defect in FIGNL1-depleted DR-GFP U2OS cells. (H) Amino acids 121–240 deletion mutant could not rescue IR hypersensitivity of FIGNL1-depleted cells. Data are presented as mean ± SD from three different experiments. Ctrl, control; IB, immunoblotting.