Skip to main content
. 2013 Jun 10;110(26):E2420–E2427. doi: 10.1073/pnas.1309354110

Fig. 2.

Fig. 2.

PS increases SIRT1 stability via CaMKKβ phosphorylation at Ser-27 and Ser-47. Immunoblots of HUVECs pretreated with CHX (0.1 mg/mL) for 30 min before PS or kept under static conditions for the indicated time (A and B), transfected with or without control siRNA or CaMKKβ siRNA and then PS for 8 h (B and C), and transfected with control siRNA or CaMKKβ siRNA and then PS or static conditions for 8 h (D). (E) Immunoblots of lysed SIRT1−/− MEFs transfected with plasmid encoding SIRT1-S27AS47A or SIRT1-S27DS47D and then treated with CHX for the indicated times. The antibodies against targeted proteins are indicated. The plots below the immunoblots summarize the mean ± SEM results from three independent experiments. *P < 0.05.