Fig. 5.

CaMKKβ and SIRT1 are involved in the regulation of antioxidative and anti-inflammatory genes in mouse aorta. (A) Immunoblots of tissue lysates from the aortic arch (AA) and the thoracic aorta (TA) isolated from CaMKKβ^+/+ mice and their CaMKKβ^−/− littermates performed with indicated antibodies. Bar graphs to the right summarize the means ± SEM from six mice in each group. (B) Levels of SOD1, SOD2, catalase, NRF1, Nrf2, KLF2, PGC1α, eNOS, ICAM-1, VCAM-1, E-selectin, and MCP-1 mRNA (relative to GADPH) in TA from CaMKKβ^−/− and their CaMKKβ^+/+ littermates. The results summarize the means ± SEM from 15 mice in each group. *P < 0.05.