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Surgical approach for intracerebroventricular injection of E15 fetal mouse brains. Following exposure of the uterine horns, individual fetal brains were injected on the left side with 5 μl of lactated Ringer's solution containing 5 × 109 viral particles of either rAAV5-GFP or rAAV9-GFP, or lactated Ringer's alone (mock). GFP, green fluorescent protein; rAAV, recombinant adeno-associated virus.
