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. 2013 Jun 25;2(6):e101. doi: 10.1038/mtna.2013.27

Figure 5.

Figure 5

GFP transgene expression in C57BL/6 mice. (a) Minimal transduction of choroid plexus (CP) epithelia was evident in E17 C57BL/6 mouse fetuses after intracerebroventricular administration on E15 of either rAAV5-GFP or rAAV9-GFP. (b) Intense expression of GFP in CP epithelia was evident by P2 and P22 compared with E17 and to CD-1 mice at P2 and P22 (Figure 2c). CPL: CP-lateral ventricle; CP4: CP-fourth ventricle. Bars, 100 μm. Densitometric quantitation of GFP expression in C57BL/6 mice on E17, P2, and P22 in (c) lateral and (d) fourth cerebral ventricles. As in CD-1 mice (Figure 2d,e), expression in C57BL/6 appears generally the highest on P2 (of the timepoints evaluated). GFP expression mediated by rAAV9 was statistically higher than by rAAV5 in fourth ventricle CP epithelia on P2 (in d). GFP, green fluorescent protein; rAAV, recombinant adeno-associated virus.