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. 2013 Jun 18;2(6):e100. doi: 10.1038/mtna.2013.30

Figure 4.

Figure 4

MiR-21 and PTEN/PDCD4 expression in U87 glioblastoma and GL261 glioma cells following incubation with chlorotoxin (CTX)-coupled liposomes encapsulating anti-miR-21 oligonucleotides. (a,b) MiR-21 and (c) PTEN/PDCD4 mRNA expression levels in (a) U87 and (b) GL261 cells, 48 hours after cell incubation with CTX-coupled liposomes encapsulating anti-miR-21 or scrambled oligonucleotides (n = 3). MiR-21 expression levels, normalized to the reference snord44 (human) or snord110 (mouse), and PTEN and PDCD4 expression levels, normalized to the reference HPRT1, are presented as relative expression values to control untreated cells. (d) Representative gel showing PTEN and PDCD4 protein levels in U87 (upper panel) and GL261 (lower panel) cells 48 hours after cell incubation with CTX-coupled liposomes encapsulating anti-miR-21 or scrambled oligonucleotides (n = 3). (e) Quantification of PTEN and PDCD4 bands observed in d, corrected for individual α-tubulin signal intensity. Results are presented as PTEN and PDCD4 expression levels relative to control. Values are presented as means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 to cells incubated with a similar amount of CTX-coupled liposomes encapsulating scrambled oligonucleotides.