FIG. 1.

Overview of the rounds performed and intermediate steps during the cell systematic evolution of ligands by exponential enrichment (SELEX) procedure. (A) The net aptamer binding affinities following the different cell SELEX rounds are illustrated. The intermediate steps were gel elution, indicated by the black arrows, negative counterselection with BICR56, indicated by the gray flash, and negative counterselection with PBMCs, indicated by the white flash. (B) Fluorescence activated cell sorting (FACS) histograms illustrating the obtained binding affinities during the different rounds of the SELEX process. PBMCs, peripheral blood mononuclear cells. Color images available online at www.liebertpub.com/nat