FIG. 11.

Quantification of the calcium phosphate precipitates in the aptamer 74-positive and aptamer 74-negative fractions and gene expression analysis of osteogenic marker genes directly after the fluorescent cell sorting of JPCs that were osteogenic induced for 5 days. (A) Representative Alizarin red staining of aptamer 74-positive versus -negative cells after day 30 of osteogenic differentiation as compared to undifferentiated JPCs. Abbreviations: co,untreated; OB, osteogenic differentiation. (B) Photometric quantification of the secreted calcium phosphate precipitates in aptamer 74-sorted cells. (C) Representative van Kossa stainings of aptamer 74-positive versus -negative cells after day 30 of osteogenic induction compared to undifferentiated JPCs. (D) Gene expression analyses of the aptamer 74-positive versus -negative cell fractions. Induction factors of gene expression levels in aptamer 74-positive versus -negative cells are indicated in the diagram. Color images available online at www.liebertpub.com/nat