Table 1.
Application | Sequence | |
---|---|---|
Starting library | (a) | 5′-GGGAGACAAGAATAAACGCTCAA-N40-TTCGACAGGAGGCTCACAACAG GC-3′ |
Modified PCR primers | (b) | 5′phosphate-GCCTGTTGTGAGCCTCCTGTCGAA-3′ |
(c) | FITC-C18 - GGGAGACAAGAATAAACGCTCAA-3′ | |
Gel elution | (d) | A20 - C18 -5′ GCCTGTTGTGAGCCTCCTGTCGAA-3 |
Coupling of aptamer 74 to Dynabeads or western blotting | (e) | 5′-biotin-C18- GGGAGACAAGAATAAACGCTCAACAAATGGGTGGGTGTGGTGGGTGTGAAGGTGCGAGTTGATTCGACAGGAGGCTCACAACAGGC |
Coupling aptamer 74 to gold electrodes | (f) | 5′-thiol-C18- GGGAGACAAGAATAAACGCTCAACAAATGGGTGGGTGTGGTGGGTGTGAAGGTGCGAGTTGATTCGACAGGAGGCTCACAACAGGC |
FITC, fluorescein isothiocyanate; PCR, polymerase chain reaction.