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. 2013 Feb;23(1):44–61. doi: 10.1089/nat.2012.0349

Table 1.

List of Oligonucleotides Used

Application   Sequence
Starting library (a) 5′-GGGAGACAAGAATAAACGCTCAA-N40-TTCGACAGGAGGCTCACAACAG GC-3′
Modified PCR primers (b) 5′phosphate-GCCTGTTGTGAGCCTCCTGTCGAA-3′
  (c) FITC-C18 - GGGAGACAAGAATAAACGCTCAA-3′
Gel elution (d) A20 - C18 -5′ GCCTGTTGTGAGCCTCCTGTCGAA-3
Coupling of aptamer 74 to Dynabeads or western blotting (e) 5′-biotin-C18- GGGAGACAAGAATAAACGCTCAACAAATGGGTGGGTGTGGTGGGTGTGAAGGTGCGAGTTGATTCGACAGGAGGCTCACAACAGGC
Coupling aptamer 74 to gold electrodes (f) 5′-thiol-C18- GGGAGACAAGAATAAACGCTCAACAAATGGGTGGGTGTGGTGGGTGTGAAGGTGCGAGTTGATTCGACAGGAGGCTCACAACAGGC

FITC, fluorescein isothiocyanate; PCR, polymerase chain reaction.