FIG. 3.

Effects of truncated form of WT1 expression in SmartDC: Identity and potency markers for SmartDC/tWT1. (a) Representative example of immunophenotypic marker analyses of SmartDC/tWT1 in comparison to ConvDC and SmartDC (day 7). Dotted lines indicate the isotype controls for the respective antibody staining, and the full lines indicate the relevant monoclonal antibodies. Numbers indicate the percent positive cells for the appropriate immunophenotypic marker. (b) Comparison of immunophenotypic identity markers in ConvDC, SmartDC, and SmartDC/tWT1—(i) HLA-DR and (ii) CD86. Histogram bars represent the data obtained from experiments performed with cells from three different donors (n=3). (c) Endogenously secreted identity cytokines; GM-CSF and IL-4 and potency cytokine markers; IL-8, TNF-α, IL-6, and MCP-1 analyzed from the supernatants obtained from SmartDC and SmartDC/thWT1 cultures (day 7). Data represent the average of experiments performed with cells obtained from three different donors (n=3). Error bars indicate SEM.