FIG. 3.

TK-ts13 cells infected with SeVs expressing TRIM5α lacking the PRYSPRY domain [SPRY(−); white circles], MT4-derived human TRIM5α (249D; black squares), or human TRIM5α (249G; black triangles) were exposed to green fluorescence protein (GFP)-expressing HIV-1 vector based on BH10 (A) or NL4-3 (B). (C) Cf2Th cells infected with SeVs were exposed to an HIV-2 vector based on ROD. GFP-positive cells were counted by a flow cytometer. Data points are means for triplicate samples with SD. *^,** The statistically significant differences, p<0.05 and p<0.001, respectively, in unpaired t test between human TRIM5α (249D) and human TRIM5α (249G). Representative results of two independent experiments are shown.