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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biomed Microdevices. 2012 Feb;14(1):7–16. doi: 10.1007/s10544-011-9580-0

Fig.5. Simultaneous islet perifusion and multi-channel fluorescence imaging in response to glucose and tolbutamide.

Fig.5

(a) A representive fluorescence record of [Ca2+]c and ΔΨM of a mouse islet in response to 14 mM glucose (n=15-20 islets) (b) A representive trace of the insulin kinetics from 20 mouse islets in response to 14 mM glucose (n=3). (c) A representive fluorescence record of [Ca2+] c and ΔΨM of a mouse islet in response to 250 μM tolbutamide (n=15-20 islets). (d) A representive trace of the insulin kinetics from 20 mouse islets in response to 250 μM tolbutamide (n=3). The signals were normalized against basal intensity established before the stimulation and expressed in percentage changes. Flow rate of delivered insulin secretagogues was set at 250 μL/min. The hashmarks indicated time of insulin secretagogues stimulation.