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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Alzheimers Dis. 2013;33(3):797–805. doi: 10.3233/JAD-2012-121426

Figure 4.

Figure 4

Daidzein increases AβPP expression in primary cultured neurons. (A) Primary wild type neurons were treated with 0, 5, 50 or 5,000 ng/mL daidzein or genistein for 1 day prior to fixation and staining for AβPP. A minimum of 616 puncta were analyzed per treatment. The percent of AβPP expression compared to untreated controls (white bars) was plotted against treatments (gray bars). Statistical significance was determined by one-way ANOVA (daidzein: P ≤ 0.002, F = 5; genistein: P = 0.06, F = 2.4) and by student t-test analyses (all daidzein-treated samples were statistically different from untreated controls, denoted by stars, P ≤ 0.01). (B) Primary wild type neurons were treated with 5 ng/mL daidzein or genistein for 15 min, 1 hr or 4 hr prior to fixation and staining for AβPP. A minimum of 746 puncta were analyzed per treatment. Statistical significance was determined by one-way ANOVA (4 h: P ≤ 0.05, F = 3.2) and student t-test (control versus daidzein 4 h: P ≤ 0.05; control versus genistein: P ≤ 0.01). Shorter time points were not statistically significant.