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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mucosal Immunol. 2011 Oct 19;5(1):53–65. doi: 10.1038/mi.2011.47

Figure 4.

Figure 4

CD103+ dendritic cells (DCs) are required for Th2-cell differentiation. (a, b) Analysis of DCs from lymph nodes (LNs) of Ccr7−/− mice. (a) Flow cytometry plots showing percentages of CD11bhi and CD103+ DCs in mediastinal LNs of wild-type (WT) C57BL/6 and Ccr7−/− mice. (b) Histograms showing total DC numbers for each DC subset (n=5–6). Data shown are from one of the two independent experiments giving similar results. (c, d) Induction of T-cell cytokines by total LN DCs (c) or lung-resident DC subsets (d). Naive CD4+ OT-II T cells were cultured with the indicated DCs, and cytokine production from T cells was measured by enzyme-linked immunosorbent assay following elicitation by anti-CD3ε and CD28 antibodies. P-values by Student’s t-test. IFN, interferon; IL, interleukin; NS, not significant.