Figure 2.

Endothelial nitric oxide synthase expression and phosphorylation at the S1177 activator site in cerebral arteries from normal salt–fed (n = 8) and high salt–fed (n = 6) Ren1-BN congenic rats (a–c) and high salt–fed Ren1-BN congenic rats receiving an infusion of either angiotensin II (ANG II; 100ng/kg/min; n = 4) or isotonic saline vehicle (n = 5) by subcutaneously implanted osmotic minipump (d–f). Panels a and d show representative Western blots of eNOS and phospho-eNOS (S1177). Panels b and e show total eNOS expression normalized to β-actin. Panels c and f show phosphorylation of eNOS at S1177, normalized to total eNOS. All values are summarized as mean ± SEM.