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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Clin Cancer Res. 2012 Dec 4;19(3):560–570. doi: 10.1158/1078-0432.CCR-12-2334

Figure 4. Effect of Gln depletion and SLC1A5 inhibition on cell cycle progression.

Figure 4

(A) Representative bright field (20× magnification) images of A549 cells after 48 hours culturing in various growth conditions as described below. (B) DNA histograms of cell cycle phase distribution of 104 cells after 48 hours of culturing A549 cells in growth media supplemented with EGF, insulin, selenium and transferein and 2mM of L-glutamine (+Gln +Sup), or L-glutamine but no supplements (+Gln-Sup), or no L-glutamine but with supplements (−Gln+Supp) or not supplemented with neither L-glutamine not supplements or (+Gln +Sup) in that contain 5mM of GPNA.