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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Methods Enzymol. 2010;485:213–224. doi: 10.1016/B978-0-12-381296-4.00012-9
Volume (μL)
70 Deionized water
10 10× buffer (100 mM Tris buffer, pH 7.5, 1 M NaCl, 50 mM MgCl2)
2 50 mM DTT
5 Transducin (50 μM stock)
10 Opsin/visual pigment (typically, 10–50 nM stock concentration) in membranes