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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mod Pathol. 2012 Aug 10;25(12):1599–1608. doi: 10.1038/modpathol.2012.121

Table 3.

Region-specific DNA capture and sequencing performance

Cancer Designation 1 2 3 4 5 Biphasic Cancer: Low Grade Region Biphasic Cancer: High Grade Region
Pass-Filter Read Pairs 1751408 1552882 2834330 2059979 3459801 16908123 26247753
Total reads aligned 3391847 2995682 5468661 3970439 6567690 32671738 50742320
% of reads aligned 96.8 96.5 96.5 96.4 94.9 96.70% 96.70%
% of Bases on/near targets* 83.1% 79.9% 80.3% 82.9% 71.4% 85.20% 85.10%
Average target coverage 209 177 318 243 331 1758 2680
% Usable bases on target 47.9% 46.0% 45.3% 47.6% 39.3% 46.90% 45.80%
Fold enrichment 2379 2276 2247 2371 1967 2360 2316
% zero-coverage targets 1.0% 1.1% 0.8% 1.1% 0.9% 0.80% 0.80%
% of target bases with >=2x coverage 99.3% 99.3% 99.1% 99.2% 99.5% 99.50% 99.50%
% of target bases with >=10x coverage 98.3% 98.5% 98.4% 98.5% 98.9% 99.10% 99.30%
% of target bases with >=20x coverage 97.6% 97.5% 97.9% 97.7% 98.4% 98.80% 99.00%
% of target bases with >=30x coverage 96.6% 96.4% 97.5% 96.7% 98.0% 98.60% 98.80%
*

Total target length = 546,336 bases; total bait length = 814,497 bases