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Validation of novel single nucleotide variants by Sanger sequencing
| Validation Status | Number |
|---|---|
| SNVs with validation attempted | 31 |
| Sanger data confirmed Illumina data | 28 (90%) |
| Somatic mutation | 4 |
| Germline polymorphism | 22 |
| Somatic status unknown* | 2 |
Variant as detected in Illumina data was confirmed by Sanger sequencing, but the genotype in the normal DNA could not be determined with accuracy.
