Figure 3.

Gli3 Processing and Hh and FGF Signaling Are Altered in Fuz Mutants

(A) Western blot analysis of activator and repressor forms of Gli3 (Gli3-L and Gli3-S, respectively) in E9.0 embryos. Increased Gli3-L and reduced Gli3-S was detected in Fuz^−/− embryos in (A), lanes 3–5, compared to controls in (A), lanes 1 and 2. Alpha-tubulin was analyzed as a loading control.

(B) qPCR of Hh target genes Patched 1 (Ptc1) and Gli1 from E9.0 Fuz^+/+ and Fuz^−/− heads. Relative mRNA levels are normalized to β-actin. Ptc1 and Gli1 are decreased in mutants (red) compared to controls (blue). Whiskers represent maximum/minimum data values with median and quartiles represented in the box.

(C and D) Lateral views of Fgf8 expression in E9.0 embryos. Fgf8 is expressed in the midhindbrain boundary (MHB), frontonasal prominence (FNP), and the BA1 epithelium. In mutants, Fgf8 expression domains are expanded, indicated by bracket and arrowheads.

(C′ and D′) Magnified view of BA1 in control and mutant embryos. Dotted line indicates extent of maxillary compartment. Note enlarged maxillary prominence.

(E and F) Frontal view of Fgf8 expression in E10.5 embryos. Note mediolateral expansion of maxillary Fgf8 expression in the mutant, indicated by black arrowhead.

(G and H) Lateral views of Erm1 mRNA expression in E9.5 embryos. Erm1 is expressed in the MHB, FNP, and BA1. In mutants, expression of Erm1 is expanded ventrally from the MHB (yellow asterisk) and in BA1 (yellow bracket).

(G′ and H′) Magnified view of BA1. Erm1 expression is increased in mutant maxillae (yellow arrowheads).

(I and J) Lateral views of Pea3 expression in E9.5 embryos. Pea3 is expressed in the MHB, FNP, and BA1. In mutants, expression of Pea3 is expanded in BA1 (yellow bracket).

(I′ and J′) Magnified view of BA1 in control and mutant embryos. Pea3 expression is increased in mutant maxillae (yellow arrowhead).

See also Figure S2.