Table 1.

Oligodeoxynucleotide primers used in subcloning of the E. coli hotdog fold thioesterases

Target gene	Forward primer	Reverse primer
ybgC	GGCGGATCCATGAATACAACGCTGTTTCGATGGCCGG	GCGGAATTCACTGCTTAAACTCCGCGACAATAGACTTG
ybdB (entH)	GCGCGGATCCATGATCCTGCACGCGCAGGCAAAACACG	CCGGAATTCTCAGAAACGCAAGATCTGCGCCAGACTTGC
ydiI	GGCGGATCCATGATATGGAAACGGAAAATCACCCTGG	GCGGAATTCTCACAAAATGGCGGTCGTCAATCGTGACG
ybaW	GGCGGATCCATGCAAACACAAATCAAAGTTCGTGGATATCATCTCG	CCGGAATTCTTACTTAACCATCTGCTCCAGCTTTTCGCGCAATTCCCC
yciA	GGCGGATCCATGTCTACAACACATAACGTCCCTCAGGGCG	GCGGAATTCTTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGG
yigI	GGCGGATCCATGTCTGCCGTACTGACCGCTGAACAAG	GCGGAATTCTCAACCTACCATATAGGTGGCGGTGGCACTG
yiiD	GGCGGATCCATGAGCCAGCTTCCAGGGTTGTCACGGG	GCGGAATTCCTACTCTTCTTCGTTCCCGCCCTCTTCATACGG
tesB	GCGGAATTCATGAGTCAGGCGCTAAAAAATTTACTGACATTG	GCGAAGCTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGG
paaI	GGCGGATCCATGAGTCATAAGGCCTGGCAAAATGCCCATGC	GCGGAATTCTCAGGCTTCTCCTGTAATGGTGCCGCCGATGCGG