Table 1.

Dissociation constants of SecA and its amino-terminal variants at different KCl concentrations

KCl concn (mM)	Varianta	Kd, μM (mean ± SD)	Source or reference
300	SecA (no His)	3.14 ± 1.81	10
	WT SecA	3.65 ± 0.19	This study
	SecA Δ2-11	NDb	This study
	SecA-A6	ND	This study
	SecA L2A I3A	5.99 ± 0.30	This study
	SecA V9A F10A	29.50 ± 2.51	This study
	SecA L5A	11.03 ± 2.23	This study
	SecA L6A	19.48 ± 4.84	This study
200	SecA (no His)	0.28 ± 0.13	10
	SecA Δ2-11	29.87 ± 3.72	This study
100	SecA (no His)	0.014 ± 0.009	10
	SecA Δ2-11	0.13 ± 0.03	This study

^aWT SecA, SecA with the carboxy-terminal His tag; SecA Δ2-11, deletion of residues 2 through 11 from SecA (this is referred to as SecAΔ11 in some references [17, 28]); SecA-A6, mutant SecA L2A I3A L5A L6A V9A F10A.

^bND, not detectable (no dimer was detected at a concentration of up to 10 μM).