Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis by SDS-PAGE of the overexpression of the Hpa (A) and Adh (B) candidate genes in E. coli and of the purification of the His-tagged proteins. Lane M, molecular mass markers; lane 1, whole cells prior to IPTG induction; lane 2, whole cells 5 h after induction; lane 3, soluble protein fraction after ultracentrifugation (100 μg protein); lane 4, protein fraction obtained from affinity chromatography (30 μg protein).
