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Anti-TcpF immunoprecipitation in a ΔtcpA strain does not coimmunoprecipitate a 20-kDa protein. Anti-TcpF coimmunoprecipitation products of cross-linked whole-cell lysates were loaded on an SDS-PAGE gel and subjected to electrophoresis. Silver staining demonstrates an absence of the 20-kDa band in the ΔtcpA strain.
