Fig 2.

DNA binding activity of wild-type MtOGT and point-mutated proteins. (A) Band shift analysis of MtOGT (wild type [WT]), MtOGT-T15S (T15S), and MtOGT-R37L (R37L) proteins. Lane 1, 1 pmol of fluorescent double-stranded DNA probe (DNA); lanes 2 to 9, increasing amounts of protein (P) incubated in the presence of the probe at the indicated DNA/protein molar ratios. The white and black arrowheads point to the free and protein-bound probes, respectively. (B) Plot of the DNA-bound protein fractions at the DNA/protein molar ratios indicated in panel A. K, dissociation constant (μM).