Table 1.
Kinetic constants of the reaction catalyzed by MtOGT and mutated proteins
| Protein | dsDNAmet concn (μM) | Mean k (s−1) ± SD | Mean KVG (μM) ± SD | Mean KDNA (μM) ± SD |
|---|---|---|---|---|
| MtOGT | 0 | 0.12 ± 0.02 | 1.82 ± 0.4 | 0.24 ± 0.11 |
| 0.63 | 0.14 ± 0.03 | 3.53 ± 0.5 | ||
| 1.00 | 0.10 ± 0.03 | 5.29 ± 0.9 | ||
| 1.25 | 0.14 ± 0.02 | 8.68 ± 0.8 | ||
| MtOGT-T15S | 0 | 0.10 ± 0.01 | 1.73 ± 0.5 | 0.48 ± 0.26 |
| 0.63 | 0.11 ± 0.01 | 2.29 ± 0.4 | ||
| 1.00 | 0.13 ± 0.02 | 3.46 ± 0.5 | ||
| 1.25 | 0.12 ± 0.03 | 5.40 ± 0.7 | ||
| MtOGT-R37L | 0 | 0.07 ± 0.02 | 3.17 ± 0.5 | 2.16 ± 0.23 |
| 0.63 | 0.09 ± 0.02 | 4.23 ± 0.7 | ||
| 1.00 | 0.07 ± 0.01 | 4.55 ± 0.6 | ||
| 1.25 | 0.08 ± 0.02 | 5.11 ± 0.5 |