Fig 5.

SPR sensogram. (A) Sensogram showing the difference in level of interaction of analyte (HpDnaB helicase) with HpDnaG-CTD native and mutant. The amounts of analyte passed in both cases were the same: 525 nM. (B and C) Sensorgram showing the different binding affinities of native HpDnaG-CTD and mutant HpDnaG-CTD for HpDnaB helicase. The legend shows the concentration of analyte (DnaB helicase used). The mutation of a single residue, Phe534 to Ala, on the helicase-binding surface resulted in a 16-fold decrease in the measured binding affinity.