Fig 4.
ExbB S34A and W38A TMD mutants are not dominant. ExbB mutants were coexpressed with ExbD using 0.01% l-arabinose in wild-type (WT) strain W3110. All mutant proteins were overexpressed >100-fold compared to chromosomal levels, based on serial dilutions and immunoblot analysis (data not shown). Initial rates of [55Fe]ferrichrome transport were measured from triplicate samples and normalized to the results for strain W3110 (100%). Averaged percentages of at least two independent experiments are shown.