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. 2012 Jan 13;21(1-2):8–19. doi: 10.1159/000335354

Fig. 2.

Fig. 2

Chromosomal Lac reversion mutations are stimulated by I- Sce I-induced DSBs over time during starvation. a, c, e, g Cartoon of E. coli chromosome showing position of PBADI-SceI, lacI33ΩlacZ allele, (lac+1), and I- Sce I cut sites (I-sites). Arrows denote direction of transcription. b, d, f, h I-SceI-induced DSBs increase Lac+ colonies compared with cutsite-only control strains (CS). Cultures were grown at 37°C. DSB strains (•) carry both PBADISceI and one I- Sce I cut site; and CS (▪) carry I-SceI cutsite only. a, b I-site codA21. c, d I-site codA22. e, f I-site mhpA32. g, h I-site mhpA33. All cutsites except mhp33 were used by Ponder et al. [2005]. Strains used: SMR6330, SMR6329 (a, b); SMR6332, SMR6331 (c, d); SMR6326, SMR6325 (e, f); SMR6328, SMR6327 (g, h).