Table 1.
E. coli K12 strains and plasmids
| Plasmid/strain | Relevant genotype | Reference or source |
|---|---|---|
| DH5α | SuII+ recA hsdrK–mK+ | Hanahan [1983] |
| pCW15(C127) | pACYC184 derivative, expresses Tn7 transposition proteins TnsA, B, and CA225V, CamR | Stellwagen and Craig [1997] |
| pMCB40 | pBW30 derivative, miniTn7Kan donor plasmid, KanR, has R6Kλ origin that requires π protein for replication | Biery et al. [2000] |
| pRP1 | pMCB40 mini Tn7Kan(I-SceI site) donor plasmid, KanR, requires π protein for replication | Ponder et al. [2005] |
| pTGV express | pBR322-derived chromosomal expression cassette-integration vector, has PBAD and a multiple cloning site flanked by gal and bio sequences homologous to DNA surrounding att λ AmpR, Tets | Gumbiner-Russo et al. [2001] |
| MG1655 | Wild type | Blattner et al. [1997] |
| BW23474, or MB69 | endA(BT333) hsdR514 recAl ΔuidA(MluI)::pir-116 (expresses π protein for R6Kλ plasmid replication) | Biery et al. [2000] |
| FC36 | δ(lac-proB)XIII thi ara RifRF- λ- | Cairns and Foster [1991] |
| FC40 | FC36[F’ laclq lacI33ΩllacZ proAB+] | Cairns and Foster [1991] |
| K12 [F+] | Wild type [F+] | Bachmann [1972] |
| MB75 | BW23474 [pMCB40] | Biery et al. [2000] |
| SMR696 | proC zaj3053::Tn10 Δ lacX74 | Lab collection |
| SMR1316 | MG1655 RhR | Spontaneous rifampicin resistance |
| SMR1698 | MG1655 RifR proC zaj3053:Tn10 ΔlacX74 | SMR1316 × PI SMR696 |
| SMR4453 | FC40 5FCR 5FUS codA/B::Tn10dTet | FC40 × XNK1323 |
| SMR4514 | MG1655 RifR proC zaj3053:Tnl0 ΔlacX74 | SMR1698 × P1 MG1655 |
| SMR4522 | MG1655 cod::Tn10 lacq lacI33ΩlacZ | MG1655 × PI SMR4453 |
| SMR4562 | Independent construction of FC40 | McKenzie et al. [2000] |
| SMR4615 | MG1655 lacq lacI33ΩlacZ RifR | SMR4514 × PI SMR4522 |
| SMR4874 | SMR4615 lacq lacI33ΩlacZ RifR [F+] | SMR4615 mated with K12 [F+] |
| SMR5078 | recB21 recC22 sbcB15 sbcC201 hsdrK−mK+ (λ xis1 cIt857)-mono lysogen | Gumbiner-Russo et al. [2001] |
| SMR5405 | FC40 [pCW15(C127)] | Ponder et al. [2005] |
| SMR5448 | DH5Α [pTGV express] | Gumbiner-Russo et al. [2001] |
| SMR5739 | BW23474 [pRPl-13] | BW23474 × ligation mix of pMCB40 DNA with oligos |
| SMR5741 | BW23474 [pRPl-13-1] | BW23474 × digested and religated pRP1-13, selected KanR, confirmed by sequencing |
| SMR5744 | recB21 recC22 sbcB15 sbcC201 Δattλ::PBADI-SceI | Gumbiner-Russo et al. [2001] |
| SMR5746 | recB21 recC22 sbcB15 sbcC201 Δattλ::PBAD | Gumbiner-Russo et al. [2001] |
| SMR5973 | SMR4562 [F’ mhpA32::miniTn7Kan(I-SceI site)] [pCW15(C127)] | Ponder et al. [2005] |
| SMR5974 | SMR4562 [F’ mhpA33:: miniTn7Kan(I-SceI site)] [pCW15(C127)] | SMR5405 × pRPl, selected KanR, screened 5-FCR; 5-FUs, Tn orientation determined by PCR; insertion between F’ equivalents of chromosomal bp 369,382 and 369,383 |
| SMR5975 | SMR4562 [F’ codA21::miniTn7Kan(I-SceI site)] [pCW15(C127)] | Ponder et al. [2005] |
| SMR5976 | SMR4562 [F’ codA22::miniTn7Kan(I-SceI site)] [pCW15(C127)] | Ponder et al. [2005] |
| SMR6267 | SMR4615 leu::Tn10 [F+] (λ xisl cIts857) | SMR5995 × PI SMR6263 |
| SMR6278 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI | SMR6274 × PI SMR5744 |
| SMR6279 | SMR4615 ΔaraBAD567 Δattλ::PBAD | SMR6274 × PI SMR5746 |
| SMR6292 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI mhpA32::miniTn7Kan(I-SceI site) | SMR6278 × PI SMR5973 |
| SMR6293 | SMR4615 ΔaraBAD567 Δattλ::PBAD mhpA32::miniTn7Kan(I-SceI site) | SMR6279 × PI SMR5973 |
| SMR6294 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI mhpA33::miniTn7Kan(I-SceI site) | SMR6278 × PI SMR5974 |
| SMR6295 | SMR4615 ΔaraBAD567 Δattλ::PBAD mhpA33::miniTn7Kan(I-SceI site) | SMR6279 × PI SMR5974 |
| SMR6296 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI codA21::miniTn7Kan(I-SceI site) | SMR6278 × PI SMR5975 |
| SMR6297 | SMR4615 ΔaraBAD567 Δattλ::PBAD codA21::miniTn7Kan(I-SceI site) | SMR6279 × PI SMR5975 |
| SMR6298 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI codA22::miniTn7Kan(I-SceI site) | SMR6278 × PI SMR5976 |
| SMR6299 | SMR4615 ΔaraBAD567 Δattλ::PBAD codA22::miniTn7Kan(I-SceI site) | SMR6279 × PI SMR5976 |
| SMR6323 | SMR4615 ΔaraBAD567 Δattλ::PBAD [F+] | SMR6279 × SMR4874 |
| SMR6324 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI [F+] | SMR6278 × SMR4874 |
| SMR6325 | SMR4615 ΔaraBAD567 Δattλ::PBAD mhpA32::miniTn7Kan(I-SceI site) [F+] | SMR6293 × SMR4874 |
| SMR6326 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI mhpA32::miniTn7Kan(I-SceI site) [F+] | SMR6292 × SMR4874 |
| SMR6327 | SMR4615 ΔaraBAD567 Δattλ::PBAD mhpA33::miniTn7Kan(I-SceI site) [F+] | SMR6295 × SMR4874 |
| SMR6328 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI mhpA33::miniTn7Kan(I-SceI site) [F+] | SMR6294 × SMR4874 |
| SMR6329 | SMR4615 ΔaraBAD567 Δattλ::PBAD codA21::miniTn7Kan(I-SceI site) [F+] | SMR6297 × SMR4874 |
| SMR6330 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI codA21::miniTn7Kan(I-SceI site) [F+] | SMR6296 × SMR4874 |
| SMR6331 | SMR4615 ΔaraBAD567 Δattλ::PBAD codA22::miniTn7Kan(I-SceI site) [F+] | SMR6299 × SMR4874 |
| SMR6332 | SMR4615 ΔaraBAD567 Δattλ::PBADI-SceI codA22::miniTn7Kan(I-SceI site) [F+] | SMR6298 × SMR 4874 |