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Biochanin A inhibited the expression of adipogenesis-related cytokines in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an adipogenic medium (MDI) in the presence of 0.1–1 μM biochanin A. After 12 days of incubation, expression of TNFα and IL-6 mRNA was measured by RT-PCR. GAPDH expression was used for normalization. All results are expressed as the mean ± SD of three independent experiments. *P < 0.05 compared with the control.
