Figure 3.

EA-induced apoptosis in ovarian carcinoma cells. EA-treated cells were incubated at 37°C for 12 h and stained with annexin V conjugated with FITC and then analyzed by flow cytometry. Cell protein lysates from EA-treated cells were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show proteins as indicated. Protein levels were quantified and normalized using the density of the untreated control, and the Bax : Bcl-2 ratio was calculated. The apoptotic cells, the changes in apoptosis-associated proteins, and the Bax : Bcl-2 ratio of EA-treated ES-2 are shown in (a), (c), and (e), respectively, and EA-treated PA-1 are shown in (b), (d), and (f). The data reported are the averages of three independent experiments and are expressed as means ± SD. *P < 0.05.