Fig 2.

Survival responses of M. smegmatis strains to oxidative stress generated by TBHP (left) and CuOOH (right). Mycobacteria were grown to the exponential phase and exposed to oxidative stress generated by either 2 mM TBHP or 0.6 mM CuOOH. The data were analyzed by using GraphPad Prism version 5.01 and are represented as means with standard errors of the means. Two-way analysis of variance followed by a Bonferroni test was performed. The double mutant (ΔmshA ΔegtD) was more sensitive to oxidative stress than any other strain used in this study; it was significantly (P < 0.01) sensitive to oxidative stress generated by CuOOH (right) relative to the wild type and the ΔegtD mutant.