Fig 2.
Expression of Mdr1 in C. albicans strains. Total proteins extracts were prepared from SC5314 (wild-type), DSY4278 (mrr1Δ/Δ), JEY429, JEY430, JEY431, and JEY432 (transformant strains), and JEY355 (clinical isolate) as described in Materials and Methods, separated by SDS-PAGE, transferred to nitrocellulose membranes, and detected with specific antibodies. MRR1, reference allele from SC5314; MRR1V877F, MRR1 allele from JEY355; MRR1V877, MRR1 allele from DSY291, a clinical susceptible isolate. Cells were incubated without (−) or with (+) benomyl (35 μg/ml) for 30 min as indicated. Immunodetection of unspecific signals was used as a loading control as indicated. The positions of the molecular mass standards in kilodaltons are shown on the left side.