Table 2.
Primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| MRR-Xho | GATTCTCGAGAATTGGCAGCTCTTATGTTA |
| MRR-SacI | ATTGCCGAGCTCTGTTTATCAAATTGATTTC |
| MRR-SpH | CTTTGGCATGCTTGATAATGTGCCTCTAGA |
| MRR-SacII | CTATAGCCGCGGTGGCAATTGACATTTTTA |
| MRR178_SacI | AACAGAGCTCTTTACCCCAATCCAAACAC |
| MRR-SacIId | GGAAAAAACCGCGGAACGATATACTACATACATC |
| MRR1-1 | GCTATTAATTCCTATTCC |
| ZCF36SEQ7R | CACTCAACGTAATAGTGACTTC |
| MRR1-8 | CAG AGC CCC AAG TTC GG |
| ZCF36SEQ7 | GAAGTCACTATTACGTTGAGTG |
| ZCF36SEQ1 | ATTACAATGTGTCCCACACAGG |
| MRR1-2 | CAACACTGCCTTTAGGGTTGA |
| ZCF36SEQ2 | CAGTTTACTTTATCCATTTATGCC |
| ZCF36SEQ6 | CATCCTTGTATTCCGTTTCACC |
| ZCF36SEQ3 | AGTTCCATTTATAGAAGAAGGC |
| ZCF36SEQ8 | CTTGATTGATAAGAGTTTGGATC |
| MRR1-3 | TCGTAATATGCCAGTGAAAATG |
| ZCF36SEQ4 | GTTGGAATTGCAGCTGTATCC |
| MRR-SacIId | GGAAAAAACCGCGGAACGATATACTACATACATC |
| ERG11_KpnI | GAGCATGGGTACCGGCGCGCGATTGTACGTGG |
| ERG11_XhoI | GTAACGCTCGAGTGAACAAGGTTGGGTAGTAA |
| ERG11-P3 | CTTACACTCTATGGACA |
| ERG11-ORF-F | ATTGTTGAAACTGTCATTG |
| ERG11-7 | CAGCAGAAACATCAGATA |
| ERG11-2 | CATGGGGTTGCCAATGTT |
| ERG11-6 | GAGCAAATGAACGGTCAA |
| ERG11-5 | CATATGCATTCTGAGAGT |
| ERG11-3B | CCCATTAAGAATCCCTGAA |
| ERG11-4 | CTGCTGGTTCAGTAGGTA |
| CtERG3-KpnI | TCTTGGTACCAGTATTACAGTGCACACAC |
| CtERG3-XhoI | AATCTCGAGGCTACTTTCATATCAAACGC |
| CtERG_1504 | TGTGGGACAGATTAGGTAG |
| CtERG_1263 | TTTGCATCCCATGCTTTC |
| CtERG_1013 | GGCAACTAGAGCCATTCC |
| CtERG_778 | CAGAAATCTACGGTTTAGC |
| CtERG_1502C | AGTGGTGAATTGACCATAG |
| CtERG_1207C | GGCCAATGTAACCATCTG |
| CtERG_1014C | CCAAACCGATTTCCAAAG |
| CtERG_712C | GCAAGGGAGAAATTTGAAG |
| CtERG11-ApaI | ACAATGGGCCCAAAACACGGTGAAGAAAT |
| CtERG11-XhoI | TTTTCTCGAGTGGTTGAAAAATTTTCTG |
| CtERG_401C | CATCAATGGCAGTATCAAC |
| CtERG_1113 | ACGCTGCTCAAAGAAAG |
| CtERG_1121C | GAGCAGCGTCACGTCTC |
| CtERG_1490 | CACATGCCATTGCATTC |
| CtERG_1499C | ATGGCATGTGCATTCTC |
| CtERG_1759 | ATTCGGTGGTGGTAGAC |
| CtERG_1920C | GCAGGTTCTAATGGTAAGG |
| CtERG_732C | TGGGTATAAGCTTCTTCAG |
| ERG11-ORF-F | ATTGTTGAAACTGTCATTG |
| ERG11-ORF-R | CCCCTAATAATATACTGATCTG |
| ACT1-ORF-F | GCATCACACTTTTTACAAT |
| ACT1-ORF-R | AAACATAATTTGAGTCATCTTT |
| ERG11-P2a | TTTGTCCCTTAGTGTTACACA |
| ACT1-P2a | TTGCTCCAGAAGAACATCCAGT |
| CtERG11_PyesF | ACTACTAGCAGCTGTAATACGACTCACTATAGGGAATATTAAGCTTATGGCTATTGTTGATACTGCCATT |
| CtERG11_PyesR | AGGGTTAGGGATAGGCTTACCTTCGAAGGGCCCTCTAGACTCGAGAACCATACAAGTATCTCTCTTTTCC |
| CtERG3-NcoI-NotI | CAAGGCGGCCGCCCATGGTTTGATCTTAGTAGTAG |
| CtERG3-NotI | ACAAGCGGCCGCGGTAATAAAATACAATCAAG |
| CtERG3-SacI | ATCTGAGCTCAAGTTAAAAATTTAACTC |
a
These primers were labeled with 6-FAM ( 6-carboxyfluorescein) at the 5′ end and TAMRA (tetramethylrhodamine) at the 3′ end and were used as TaqMan probes in qRT-PCR with primers ERG11-ORF-F/ERG11-ORF-R and ACT1-ORF1-F/ACT1-ORF-R.