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Analysis of trfA mRNA levels in glycopeptide-susceptible and GISA paired clinical strains. Steady-state levels of trfA transcripts were determined by qRT-PCR and normalized to 16S rRNA. All GISA mRNA levels (black bars) were compared to the corresponding glycopeptide-susceptible strains (white bars). The values represent the means and standard errors of the mean (SEM) of three independent experiments. *, results significantly different by Student's two-tailed t test (P < 0.05).
