Fig 6.

Effect of oxacillin on spx mRNA levels. (A) Steady-state levels of spx and hu transcripts were determined by qRT-PCR and normalized to 16S rRNA in strain ISP794 compared to oxacillin-treated (oxa) ISP794 cells. The values represent the means and SEM of three independent experiments. (B) Western blot analysis of total soluble protein extracts (75 μg) of S. aureus ISP794 or the same strain treated with oxacillin loaded in SDS-15% acrylamide gels. Spx protein (13 kDa) was detected using rabbit polyclonal anti-S. aureus Spx antibody as previously described (10).