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Metabolic activity of persisters. (B and C) CFU were determined at the indicated time points during the antibiotic treatment. RSG staining did not affect the persister levels (P = 0.58 for ampicillin persisters at the 5-h time point and 0.52 for ofloxacin persisters at the 5-h time point, using the t test). Persister frequencies (D) as well as fractions (E) were quantified after 5 h of antibiotic treatment of FACS-sorted cells from regions A, B, C, and D, based on RSG staining (A).
