Fig 5.

2D FACS sorting. (A) Unstained exponential-phase MO001 cells. (B) RSG-stained MO001 cells. (C and D) CFU were determined at the indicated time points during the antibiotic treatment. Persister frequencies (E) as well as fractions (F) were quantified after 5 h of antibiotic treatment of FACS-sorted cells from the NGI, NGII, GI, and GII regions (B). There was no significant difference in persister frequencies between NGI and NGII (P = 0.77 for ampicillin samples and P = 0.09 for ofloxacin samples, using the t test) or between GI and GII (P = 0.45 for ampicillin samples and P = 0.86 for ofloxacin samples, using the t test).